Brucella Ag Test Kit | |
Catalog nhamba | RC-CF10 |
Summary | Kuonekwa kweantigen chaiyo yeBrucella mukati memaminitsi gumi |
Principle | Imwe-nhanho immunochromatographic assay |
Detection Targets | Brucella antigen |
Muenzaniso | Canine, bovine uye Ovis Yese Ropa, Plasma kana Serum |
Nguva yekuverenga | 10 ~ 15 maminitsi |
Sensitivity | 91.3 % vs. IFA |
Zvakananga | 100.0% vs. IFA |
Muganho Wekuonekwa | IFA Titer 1/16 |
Quantity | 1 bhokisi (kiti) = 10 zvishandiso (Yega yekurongedza) |
Zviri mukati | Test kit, Tubes, Disposable droppers |
Kuchenjerera | Shandisa mukati memaminitsi gumi mushure mekuvhura Shandisa huwandu hwakakodzera hwemuenzaniso (0.01 ml yedonhwe) Shandisa mushure me15 ~ 30 maminetsi kuRT kana akachengetwa pasi pekutonhora Tarisa mhinduro dzebvunzo sedzisina basa mushure memaminitsi gumi |
The genus Brucella inhengo yemhuri Brucellaceae uye inosanganisira gumi mhando idiki, non-motile, non-sporing, aerobic, gram-negative intracellular coccobacilli.Iwo catalase, oxidase uye urea positive mabhakitiriya.Nhengo dze genus dzinogona kukura pamidhiya yakafumiswa seropa agar kana chokoreti agar.Brucellosis inozivikanwa zvikuru zoonosis, iripo mumakondinendi ese, asi ine kupararira kwakasiyana-siyana uye chiitiko, mumhuka uye vanhu.Brucella, sefacultative intracellular parasites, inotora mhando dzakawanda dzemhuka dzemagariro nenzira isingagumi, zvichida yekusingaperi, zvichida kwehupenyu hwavo hwose.
Brucella colony chitarisiko
Mhuka dzeBrucella dzinotapuriranwa pakati pemhuka nekusangana nebepa, chibereko, zvinwiwa zve "fetus" uye kubuda kwemukati memukadzi.mhuka ine utachiona.Mazhinji kana ese marudzi eBrucella anowanikwawo mumbeu.Murume anogona kuburitsa zvipenyu izvi kwenguva yakareba kana kweupenyu hwose.Mamwe marudzi eBrucella akaonekwawo mune mamwe mavhinga uye mabudiro anosanganisira weti, tsvina, hygroma fluid, salvia, mukaka uye mumhino uye ocular secretions.
Ecology yezoonotic Brucella kutapukira
Mumombe
Hapana nzira inoshanda yekuziva mhuka dzine utachiona nechitarisiko chadzo.Zviratidzo zvinonyanya kuoneka mumhuka dzine mimba kubvisa pamuviri kana kuberekwa kwemhuru dzisina simba.Kugadzirwa kwemukaka kunogona kudzikiswa kubva pakuchinja kwenguva yekuyamwisa kunokonzerwa nekubvisa pamuviri uye kunonoka kubata pamuviri.Zvimwe zviratidzo zvebrucellosis zvinosanganisira kuderera kuri pachena kwembereko pamwe nekunamba kwakashata, kuchengetwa mushure mekuzvarwa nehosha dzechibereko, uye (dzimwe nguva) kuwedzera, majoini eArthritic.
Mumbwa
Muimbwa, mabhakitiriya eBrucellosis anowanzo kudzika munhengo dzesikarudzi uye lymphatic system, asi zvinokwanisika kuti ipararire kuitsvo, maziso uye intervertebral disc zvakare.Apo Brucellosis inokonzera intervertebral disc, chigumisiro chinonzi discopondylitis.Mune imbwa, zviratidzo kubva kunhengo dzekubereka zvinowanzoitika.Imbwa dzechirume dzinogona semuenzaniso kuita scrotal uye testicular kuzvimba, nepo imbwa dzechikadzi dzinogona kuita miscarriage.Fivha haina kujairika, asi marwadzo anokonzerwa neBrucellosis anogona kuita kuti imbwa ishaye simba.Kana chirwere ichi chichipararira kune itsvo, maziso kana intervertebral disc zviratidzo zvinogona kutanga kuratidza kubva kune nhengo idzi.
Munguruve
Nguva iri pakati pehutachiona uye kuonekwa kwekliniki zviratidzo zvechirwere inogona kubva pavhiki imwe kusvika kumwedzi miviri.Zviratidzo zvekuti danga rabatwa nechirwere ndezviya zvekutadza kubereka - kubvisa pamuviri, kudzokera kubasa mushure mekusangana uye kuberekwa kwenguruve dzisina simba kana kufa.Dzimwe nguruve dzinogona kuita hutachiona hwechibereko uye kuratidza kubuda kwesikarudzi.Nguruve dzine utachiona dzinogona kuita machende akazvimba.Vese varume nevakadzi vanogona kukamhina nekuzvimba majoini uye/kana kuita zviratidzo zvekusashanda pamwe nekuremara kwegumbo reshure.
1.Isolation uye kuzivikanwa kwemumiririri
Rudzi rweBrucella runogona kuwanikwa kubva munyama nekuvanzika kwakawanda, kunyanya mbesa dze "fetus", kubuda kwemukadzi, mukaka(kana kubuda kwezamu), urume, arthritis yezvinwiwa zvehygroma, nemudumbu, spleen nemapapu kubva ku "fetus" dzakabviswa pamuviri.Mazhinji emhando dzeBrucella kubva kumakoloni mukati memazuva mashoma pane yakasarudzika midhiya.Kana mahwendefa achionekwa masikati kuburikidza nepakati pepakati, mapoka aya anopenya uye ruvara rweuchi hwakachenuruka.Kana ukatariswa kubva kumusoro, makoroni anoita seakakombama uye akachena separera.Gare gare makoroni anova akakura uye akasviba zvishoma.
2.Nucleic acid Method
PCR chishandiso chiri nyore chekuongorora brucellosis.Mazhinji PCR- assays akagadzirwa kuti azivikanwe yeBrucella kuvandudza kugona kwekuongorora.Iyo genus-chaiyo PCR assay yakakwana kune nyore kuzivikanwa kweBrucella.
3.Serological diagnosis
Kune akawanda serological bvunzo.Serological bvunzo dzinowanzo shandiswa kuyedza mombe imwe neimwe kana mombe dzinosanganisira buffered Brucella antigen test, complement fixation, indirect or competitive enzyme-linked immunosorbent assays(ELISA) uye fluorescence assay.