Summary | Kuonekwa kwekutapurira antibody kurwisa inotapukira bursa yeFabricius virus muhuku serum |
Detection Targets | Chicken infectious bursal disease virus antibody |
Muenzaniso | Serum
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Quantity | 1 kit = 192 Muedzo |
Kugadzikana uye Kuchengetedza | 1) Mareagents ese anofanirwa kuchengetwa pa2 ~ 8 ℃.Usaomesa nechando. 2) Sherufu yehupenyu ndeye 12 mwedzi.Shandisa zvese reagents zuva risati rapera pakiti.
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Chirwere chebursal chinotapukira(IBD), inozivikanwawo sechirwere cheGumboro, infectious bursitis uye infectious avian nephrosis, chirwere chinotapukira chevadiki.hukuuye matoki anokonzerwa neInfectious bursal disease virus (IBDV), inozivikanwa naimmunosuppressionuye kufa kazhinji pamavhiki matatu kusvika matanhatu ezera.Chirwere ichi chakatanga kuwanikwa mukatiGumboro, Delawaremuna 1962. Yakakosha mune zveupfumi kuindasitiri yehuku pasi rese nekuda kwekuwedzera kubatwa nezvimwe zvirwere uye kukanganiswa kwakashata nekubudirira.vaccination.Mumakore achangopfuura, marudzi ane hutachiwana eBDV (vvIBDV), achikonzera kufa kwakanyanya muhuku, akabuda muEurope.Latin America,South-East Asia, Africa uyeMiddle East.Utachiona hunoitwa nenzira yeoro-fecal, shiri yakabatwa ichiburitsa huwandu hwehutachiona kwemavhiki maviri mushure mekutapukirwa.Chirwere ichi chinotapuriranwa zviri nyore kubva kuhuku dzine utachiona kuenda kuhuku dzine hutano kuburikidza nekudya, mvura uye kubata pamuviri.
Kiti inoshandisa nzira yemakwikwi eELISA, pre-packaged infectious bursal virus virus VP2 protein pamicroplate, uye kukwikwidza neanti-VP2 protein antibody muserum yechikamu chakasimba chevector uchishandisa anti-VP2 protein monoclonal antibody.Pakuedzwa, mushonga unodzivirira muviri wemonoclonal kuti uongororwe uye puroteni inorwisa-VP2 inowedzerwa, uye mushure mekuiswa, kana sample ine huku infectious bursal disease virus VP2 protein-specific antibody, inosunga kune antigen pandiro yakavharwa.Nokudaro kuvhara kusungirirwa kwe-anti-VP2 protein monoclonal antibody kune antigen, mushure mekugeza kubvisa anti-unbound antibody nezvimwe zvikamu;wobva wawedzera anti-mouse enzyme-yakanyorwa yechipiri antibody kuti inyatsosunga kune antigen-antibody complex pane yekuona ndiro;Iyo isina kusungwa enzyme conjugate inobviswa nekugeza;iyo TMB substrate inowedzerwa kune microwell kuti igadzire ruvara, uye kukosha kwekunyudza kwesample kwakabatana zvisina kunaka nezviri mukati meiyo anti-VP2 protein antibody irimo, nekudaro kuzadzisa chinangwa chekuona iyo anti-VP2 protein antibody mumuenzaniso.
Reagent | Volume 96 Miedzo/192Miedzo | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Instruction | 1 pcs |