Catalog nhamba | RC-CF31 |
Summary | Kuonekwa kweCanine Dirofilaria immitis antigens, Anaplasma antibodies, E. canis antibodies uye masoja ekudzivirira chirwere eLSH mukati memaminitsi gumi. |
Principle | Imwe-nhanho immunochromatographic assay |
Detection Targets | CHW Ag Dirofilaria imitis antigens Anapalsma Ab : Anaplasma antibodiesE. canis Ab : E. canis masoja ekudzivirira chirwere LSH Ab : L. chagasi, L. infantum, and L. donovani antiboies |
Muenzaniso | Canine Yese Ropa, Plasma kana Serum |
Nguva yekuverenga | Maminitsi gumi |
Quantity | 1 bhokisi (kiti) = 10 zvishandiso (Yega yekurongedza) |
Zviri mukati | Test kit, Buffer bhodhoro, uye Disposable dropper |
Storage | Tembiricha yemumba (pa2 ~ 30 ℃) |
Kupera | Mwedzi 24 mushure mekugadzira |
Kuchenjerera | Shandisa mukati memaminitsi gumi mushure mekuvhuraShandisa huwandu hwakakodzera hwemuenzaniso (0.01 ml yedonhwe) Shandisa mushure me15 ~ 30 maminetsi kuRT kana akachengetwa pasi pekutonhora Tarisa mhinduro dzebvunzo sedzisina basa mushure memaminitsi gumi |
Honye dzemoyo dzevakuru dzinokura masendimita akati wandei pakureba uye dzinogara mumapulmonary arteries uko dzinogona kuwana chikafu chakakwana.Iwo maworms mukati metsinga anokonzera kuzvimba uye anoita hematoma.Mwoyo, ipapoka, unofanira kupomba kakawanda kupfuura kare sezvo honye dzemwoyo dzinowedzera munhamba, dzichivharira tsinga.
Kana utachiona huchidzikira (kupfuura 25 heartworms mumbwa ine 18 kg), honye dzemoyo dzinopinda muatrium chaidzo, dzichivharira kubuda kweropa.
Kana nhamba yehonye yemoyo inosvika kupfuura makumi mashanu, vanogona kutora
atriums uye ventricles.
Kana ikabatwa nehonye dzemwoyo dzinopfuura 100 muchikamu chakarurama chemwoyo, imbwa inorasikirwa nekushanda kwemwoyo uye pakupedzisira inofa.Izvi zvinouraya
chiitiko chinonzi "Caval Syndrom."
Kusiyana nezvimwe zvipembenene, mhungu inoisa tupembenene tuduku tunonzi microfilaria.Microfilaria iri mumosquito inofamba ichipinda mumbwa apo mosquito inosveta ropa kubva pambwa.Iwo mahonye emoyo anogona kurarama ari muhosi kwemakore maviri anofa kana akasaenda kune mumwe muenzi mukati menguva iyoyo.Zvipembenene zvinogara mumbwa ine pamuviri zvinogona kutapurira embryo yayo.
Kuongorora kwekutanga kwehonye dzemoyo kwakakosha zvikuru mukuabvisa.Honye dzemoyo dzinopfuura nematanho akati wandei akadai seL1, L2, L3 kusanganisira nhanho yekutapurirana kuburikidza neumhutu kuita honye dzemoyo dzevakuru.
Microfilaria mumosquito inokura kuita L2 uye L3 tupukanana tunokwanisa kubata imbwa mumavhiki akati wandei.Kukura kunoenderana nemamiriro ekunze.Tembiricha yakanaka yeparasite inodarika 13.9 ℃.
Kana umhutu hune utachiona hukaruma imbwa, microfilaria yeL3 inopinda muganda rayo.Muganda, microfilaria inokura kuita L4 kwe1 ~ 2 mavhiki.Mushure mekugara muganda kwemwedzi mitatu, L4 inokura kuita L5, iyo inofamba muropa.
L5 sechimiro chemoyo chevanhu vakuru chinopinda mukati memwoyo uye pulmonary arteries umo 5 ~ 7 mwedzi gare gare heartworms inoisa zvipembenene.
Nhoroondo yechirwere uye data yekiriniki yembwa inorwara, uye nzira dzakasiyana-siyana dzekuongorora dzinofanira kufungwa pakuongorora imbwa.Semuenzaniso, X-ray, ultrasound scan, kuongorora ropa, kuonekwa kwe microfilaria uye, mumatambudziko akaipisisa, autopsy inodiwa.
Serum kuongorora;
Kuonekwa kwemasoja ekudzivirira chirwere kana maantigen muropa
Antigen kuongorora;
Izvi zvinotarisa pakuona chaiwo maantigen echikadzi chemoyo wormhorms.Kuongororwa kunoitwa muchipatara uye kubudirira kwayo kwakakwirira.Matest kits anowanikwa pamusika akagadzirirwa kuona 7 ~ 8 mwedzi-yakakura heartworms kuitira kuti heartworms vaduku kudarika 5 mwedzi zvakaoma kuona.
Utachiona hweheartworms hunobudirira kuporeswa muzviitiko zvakawanda.Kuti ubvise honye dzese, kushandisa mishonga ndiyo nzira yakanakisa.Kukurumidza kuonekwa kwemoyo wemwoyo kunomutsa kubudirira kwehutano.Zvisinei, munguva yekupedzisira yehutachiona, kukanganisa kunogona kuitika, zvichiita kuti kurapwa kuve kwakaoma.
Utachiona hweAnaplasma phagocytophilum (hwaimbova Ehrilichia phagocytophila) hunogona kukonzera utachiona mumhando dzemhuka dzakawanda kusanganisira vanhu.Chirwere ichi chiri mumhuka dzinodya chikafu chinodaidzwa zvakare kuti tick-borne fever (TBF), uye chave nemakore anodarika mazana maviri chichizivikanwa.Utachiona hwemhuri Anaplasmataceae igiramu-negative, nonmotile, coccoid kune ellipsoid organisms, inosiyana mukukura kubva pa 0.2 kusvika 2.0um dhayamita.Iwo anosungirwa maaerobes, asina nzira yeglycolytic, uye ese anosungirwa intracellular parasites.Mhando dzese dzerudzi rweAnaplasma dzinogara mu membrane-lined vacuoles mune immature kana mature hematopoietic masero emammalian host.A phagocytophilum inobata neutrophils uye izwi rekuti granulocytotropic rinoreva neutrophils ane utachiona.Kashoma zvipenyu, zvakawanikwa mune eosinophils.
Anaplasma phagocytophilum
Zviratidzo zvekiriniki zvakajairika zvecanine anaplasmosis inosanganisira kupisa kwakanyanya, kuneta, kuora mwoyo uye polyarthritis.Neurologic zviratidzo (ataxia, pfari uye kurwadziwa kwemutsipa) zvinogonawo kuonekwa.Anaplasma phagocytophilum chirwere hachiwanzouraya kunze kwekunge chaomeswa nezvimwe zvirwere.Kurasikirwa kwakananga, kuremara kwemamiriro ezvinhu uye kurasikirwa kwekugadzirwa kwakaonekwa mumakwayana.Kubvisa pamuviri uye kukanganisa spermatogenesis mumakwai nemombe kwakanyorwa.Hukuru hwehutachiona hunopesvedzerwa nezvinhu zvakati wandei, senge akasiyana eAnaplasma phagocytophilum anosanganisirwa, mamwe hutachiona, zera, immune system uye mamiriro emuenzi, uye zvinhu zvakaita semamiriro ekunze uye manejimendi.Zvinofanira kutaurwa kuti kuratidzwa kwekiriniki muvanhu kunobva pachirwere chakapfava chakafanana nefuruwenza, kusvika kuhutachiona hunouraya.Nekudaro, hutachiona huzhinji hwevanhu hunogona kukonzera kushoma kana kusavapo kwekiriniki.
Anaplasma phagocytophilum inotapurirwa ne ixodid ticks.MuUnited States mavekita makuru iIxodes scapularis uye Ixodes pacificus, nepo Ixode ricinus yakaonekwa kuti ndiyo huru inobudisa mhepo muEurope.Anaplasma phagocytophilum inotapurirwa nevector tick idzi, uye hapana humbowo hwekutapurirana kwe transovarial.Ongororo zhinji kusvika parizvino dzakaongorora kukosha kweanoyamwisa eA. phagocytophilum uye mavekita ayo ezvikwekwe zvakatarisa pamakonzo asi chisikwa ichi chine huwandu hwakakura hwemammalian host range, huchitapurira katsi dzinopfuyiwa, imbwa, hwai, mombe, nemabhiza.
Indirect immunofluorescence assay ndiyo bvunzo huru inoshandiswa kuona hutachiona.Iyo acute uye convalescent phase serum samples inogona kuongororwa kutsvaga shanduko yakapetwa mana muantibody titer kuenda kuAnaplasma phagocytophilum.Intracellular inclusions (morulea) inoonekwa mumagranulocytes paWright kana Gimsa yakasvibiswa ropa smears.Polymerase chain reaction (PCR) nzira dzinoshandiswa kuona Anaplasma phagocytophilum DNA.
Hapana mushonga unowanikwa kudzivirira utachiona hweAnaplasma phagocytophilum.Kudzivirirwa kunoenderana nekudzivirira kuratidzwa kune chikwekwe vector (Ixodes scapularis, Ixodes pacificus, uye Ixode ricinus) kubva muchirimo kuburikidza nekudonha, prophylatic kushandiswa kweantiacaricides, uye prophylactic kushandiswa kwe doxycycline kana tetracycline paunoshanyira Ixodes scapularis, Ixodes pacide-rick-rick-rick uye Ixodes pacide-ricinus, uye endemic regions.
Ehrlichia canis tupukanana tudiki uye ane shamhu inotapurirwa nembwa yebrown chikwekwe, Rhipicephalus sanguineus.E. canis ndicho chinokonzera classical ehrlichiosis mumbwa.Imbwa dzinogona kutapukirwa neanoverengeka Ehrlichia spp.asi chinowanzokonzera canine ehrlichiosis iE. canis.
E. canis yava kuzivikanwa kuti yakapararira muUnited States yose, Europe, South America, Asia neMediterranean.
Imbwa dzine utachiona dzisati dzarapwa dzinogona kuva vatakuri vechirwere ichi kwemakore uye pakupedzisira dzinofa nekubuda ropa kukuru.
Ehrlichia canis utachiona mumbwa inokamurwa kuita nhanho nhatu;
ACUTE PHASE: Ichi chinowanzova chikamu chakapfava.Iyo imbwa ichave isina basa, isingadyi, uye inogona kunge yakawedzera lymph nodes.Panogona kuva nefivha zvakare asi kashoma chikamu ichi chinouraya imbwa.Vazhinji vanojekesa chipenyu vari voga asi vamwe vanozoenda kuchikamu chinotevera.
SUBCLINICAL PHASE: Muchikamu chino, imbwa inoita seyakajairika.The organism has sequestered in the spleen uye iri kuhwanda kunze uko.
CHRONIC PHASE: Muchikamu chino imbwa inorwara zvakare.Kusvika chikamu che60% chembwa dzine utachiona hweE. canis dzinenge dzichibuda ropa zvisina tsarukano nekuda kwekuderera kwenhamba dzemaplatelet.Kuzvimba kwakadzika mumeso kunonzi "uveitis" kunogona kuitika semugumisiro wenguva refu yekusimudzira immune system.Neurologic madhara inogonawo kuonekwa.
Definitive diagnosis yeEhrlichia canis inoda kuonekwa kwemorula mukati memonocytes pa cytology, kuonekwa kweE. canis serum antibodies ne indirect immunofluorescence antibody test (IFA), polymerase chain reaction (PCR) amplification, uye/kana gel blotting (Western immunoblotting).
Nheyo huru yekudzivirira canine ehrlichiosis ndeye kutonga kwekwekwe.Mushonga wekusarudza kurapwa kwemarudzi ese ehrlichiosis ndeye doxycycline kweinenge mwedzi mumwe.Panofanira kuva nekuvandudzwa kwekiriniki kunoshamisa mukati meawa 24-48 mushure mekutanga kurapwa mumbwa dzine acute-phase kana mild chronic-phase disease.Munguva iyi, nhamba yeplatelet inotanga kuwedzera uye inofanira kunge yakajairika mukati memazuva gumi nemana mushure mekutanga kurapwa.
Mushure mehutachiwana, zvinokwanisika kutapukirwa zvakare;kusadzivirirwa kwemuviri hakusi kwenguva refu mushure mekutapukirwa kwekare.
Nzira yakanakisisa yekudzivirira ehrlichiosis ndeyekuchengeta imbwa dzisina zvikwekwe.Izvi zvinofanirwa kusanganisira kutarisa ganda zuva nezuva nezvezvikwekwe uye kurapa imbwa nekutonga kwekwekwe.Sezvo zvikwekwe zvichitakura zvimwe zvirwere zvinoparadza, zvakadai sechirwere cheLyme, anaplasmosis uye Rocky Mountain spotted fever, zvakakosha kuti imbwa dzirambe dzisina zvikwekwe.
Leishmaniasis chirwere chikuru uye chakakomba cheparasitic chevanhu, canines uye felines.Mushonga weleishmaniasis iprotozoan parasite uye ndeye leishmania donovani complex.Chirwere ichi chinoparadzirwa zvakanyanya munyika dzinodziya uye dzinopisa dzeSouthern Europe, Africa, Asia, South America neCentral America.Leishmania donovani infantum (L. infantum) inokonzera chirwere chemhuka uye canine muSouthern Europe, Africa, neAsia.Canine Leishmaniasis chirwere chakakomba chinopfuurira systemic.Hadzisi imbwa dzese dzinoita chirwere chekiriniki mushure mekuita inoculation nemaparasite.Kukura kwechirwere chekiriniki kunoenderana nerudzi rwemhinduro yekudzivirira iyo mhuka yega yega
kurwisa utachiona.
MuCanine
Zvose visceral uye cutaneous zviratidzo zvinogona kuwanikwa panguva imwe chete mumbwa;kusiyana nevanhu, akasiyana cutaneous uye visceral syndromes haaonekwe.Zviratidzo zvekiriniki zvinosiyana uye zvinogona kutevedzera zvimwe zvirwere.Asymptomatic zvirwere zvinogonawo kuitika.Zviratidzo zvinowanzoonekwa zvinogona kusanganisira fivha (iyo inogona kupindirana), anemia, lymphadenopathy, splenomegaly, lethargy, kuderera kwekudzidzira kushivirira, kuora muviri, uye kuderera kwechido.Zviratidzo zvisinganyanyi kuzivikanwa zvinosanganisira manyoka, kurutsa, melena, glomerulonephritis, kusashanda kwechiropa, epistaxis, polyuria-polydipsia, kuhotsira, kuremara (nekuda kwepolyarthritis kana myositis), ascites, uye chisingaperi colitis.
MuFeline
Katsi hadziwanzo batwa nechirwere ichi.Mukati dzakawanda dzine hutachiwana, zvironda zvinongogumira kune maronda akachekwa, anowanzo kuwanikwa pamiromo, mhino, maziso, kana pinnae.Visceral maronda uye zviratidzo hazviwanzo.
Hupenyu denderedzwa hunopedzwa mumapoka maviri.A vertebrate host uye invertebrate host (jecha nhunzi).Nhunzi yejecha yehadzi inodya pane vertebrate host uyeinodya amastigotes.Flagellated promastigotes inokura muchipembenene.Mapromastigotes anobayiwa muvertebrate host panguva yekudyisa jecha.Mapromastigotes anokura kuita mastigotes uye anowedzera zvakanyanya mumacrophages.Kuwedzera mukati me macrophages eganda, mucosa uye viscera, kunokonzera cutaneous, mucosal uye visceral leishmaniasis zvichiteerana.
Muimbwa, leishmaniasis inowanzoonekwa nekutarisa zvakananga kwezvipembenene, uchishandisa Giemsa kana proprietary kukurumidza tsvina, mune smears kubva kune lymph node, spleen, kana bone marrow aspirates, tishu biopsies, kana ganda scrapings kubva maronda.Zvisikwa zvinogonawo kuwanikwa mumaronda emaziso, kunyanya mumagranulomas.Maastigotes akatenderedzwa kusvika kune maoval parasites, ane denderedzwa basophilic nucleus uye diki diki kinetoplast.Vanowanikwa mumacrophages kana kusunungurwa kubva kumasero akaputika.Immunohistochemistry uye polymerase chain reaction (PCR) maitiro anoshandiswa zvakare.
Mishonga inowanzoshandiswa ndeiyi: Meglumine Antimoniate yakabatana neAllopurinol, Aminosidine, uye munguva ichangopfuura, Amphotericin B. Mishonga iyi yose inoda chirongwa chemishonga yakawanda, uye izvi zvinoenderana nemamiriro emurwere uye muridzi wekubatana.Zvinokurudzirwa kuti kurapwa kwekuchengetedza kunofanira kuchengetwa neallopurinol, nokuti hazvibviri kuve nechokwadi chokuti imbwa haizodzoki zvakare kana kurapwa kwarega.Kushandiswa kwemakora ane mishonga yezvipembenene, shampoos kana sprays inoshanda kudzivirira imbwa kubva pakurumwa nejecha inofanira kuramba ichishandiswa kune vese varwere vari kurapwa.Iyo vector control ndeimwe yezvinhu zvakakosha zvekudzora chirwere.
Nhunzi yejecha inobatwa nemishonga inouraya zvipembenene yakafanana neiyo marariya vector.